中文摘要：

研究发现在碱性介质中，利血平对Cr^3＋催化的Luminol-H2O2体系的化学发光有较强的抑制作用，据此建立了流动注射化学发光抑制法测定痕量利血平的新方法。该法线性范围宽，发光信号的降低值（△I）与利血平的质量浓度在1．0×10^-8～2．0×10^-5g／mL范围内呈良好的线性关系；检出限（3σ）为7．0×10^-9g／mL；对于2．0—10^-6g／mL利血平进行11次测定，相对标准偏差为1．8％。已用于针剂中利血平的测定。通过对化学发光光谱、紫外吸收光谱的研究，对机理进行了初步探讨。

英文摘要：

Reserpine was found to inhibit chemiluminescence of the Luminol-H2O2 reaction catalyzed by Cr^3＋ in basic medium. Based on this phenomenon, a flow injection method for the determination of reserpine was developed. The method is simple, selective and sensitive with a detection limit of 7.0 × 10^-9 g/mL. It is effective to determine reserpine in the range of 1.0 ×10^-8～2.0 × 10^-5 g/mL. The relative standard deviation is 1.8% for the determination of 2.0 × 10^-4 g/mL reserpine （n = 11）. The method has been successfully applied to the determination of reserpine in the injection with satisfactory results. Furthermore, the inhibition mechanism was also discussed in this paper.