中文摘要：

以鱼肠道弧菌Vibrio ichthyoenteri为抗原免疫小鼠，用PEG法将小鼠脾细胞与SP2／0骨髓瘤细胞融合，分别采用酶联免疫吸附（ELISA）技术和间接免疫荧光抗体（EFAT）技术筛选强阳性杂交瘤细胞株，亚克隆阳性细胞株，获得5株抗鱼肠道弧菌单克隆抗体（1C6、1D8、2D7、2D10、2E3）。Western blotting分析结果显示，单抗2D10与相对分子质量为44200、36700的鱼肠道弧菌蛋白发生特异性结合；用流式细胞术（FCM）检测单抗强度，结果显示阳性荧光比例为13．63％，阴性对照荧光比例不足l％；人工感染牙鲆试验结果表明，运用该单抗能够建立鱼肠道弧菌的决速诊断方法。

英文摘要：

Five monoelonaI antibodies （MAbs） （ 1 C6,1D8,2D7,2D10,2E3 ） against Vibrio ichthyoenteri as a pathogen in marine animals were produced from hybridoma cells through Enzyme Linked Immunosorbent Assay （ELISA） and Indirect Immunofluorescenee Assay Test （IFAT） by immunised Balb/C mice. The western blotting revealed that the protein of V. ichthyoenteri was shown to have molecular weight of 44 200 and 36 700, and the flow- ing cytometry （FCM） analysis showed that the MAb 2D10 reacted with positive cells had positive percent of 13.63%, and negative percent of less than 1%. Artificial infection experiment proved that the application of mono- clonal antibody provided a method for the early diagnosis of V. ichthvoenteri.