中文摘要：

采用十二烷基肌氨酸钠（Sarkosyl）和苯甲基磺酰氟（PMSF）两种方法提取两种海洋致病性弧菌——鱼肠道弧菌Vibrio ichthyoenteri和秦皇岛弧菌Vibrio qinhuangdaorasp.nov.的外膜蛋白,并应用SDS-PAGE分析了其外膜蛋白的构成。电泳图谱显示,相对分子质量为44 000、36 000、34 000、26 000、23 000的蛋白条带为两种弧菌外膜蛋白中的共有条带。采用Western-Blotting法比较了两种弧菌外膜蛋白抗原性的异同,结果表明,鱼肠道弧菌外膜蛋白中相对分子质量分别为46 000、43 000、34 000、32 000、28 000、26 000的结构蛋白可同时与两种弧菌抗血清发生反应;秦皇岛弧菌外膜蛋白中相对分子质量分别为46 000、26 000的结构蛋白可同时与两种弧菌抗血清发生反应。两种弧菌中均存在相对分子质量为46 000、26 000的外膜蛋白,且具有共同的抗原性。

英文摘要：

The major outer membrane proteins（OMPs） were extracted from two species bacteria Vibrio ichthyoenteri,and Vibrio qinhuangdaora sp.nov.by Sarkosyl and PMSF methods,and analyzed by SDS-PAGE.The Sarkosyl electrophoresis revealed 6 common protein bands in the extracts,with the common bands with relative molecular weights of 44 000,36 000,34 000,26 000,and 23 000 in Vibrio ichthyoenteri and Vibrio qinhuangdaora sp.nov.Western-blotting were used with rabbit antiserum against Vibrio ichthyoenteri whole cells and Vibrio qinhuangdaora sp.nov whole cells to analyse difference in the strain Vibrio ichthyoenteri and strain Vibrio qinhuangdaora sp.nov.Six OMPs were detected in the strain Vibrio ichthyoenteri with molecular weights of 46 000,43 000,34 000,32 000,28 000,and 26 000,and two OMPs in the strain Vibrio qinhuangdaora sp.nov with molecular weights of 46 000,and 26 000,indicating that the OMPs with molecular weights of 46 000,and 26 000 were the common special antigen in the two bacterial strains.