中文摘要：

基于多壁纳米碳管修饰铂电极与二氧化硅溶胶-凝胶（sol-gel）固定化酶相结合的技术制备了葡萄糖氧化酶传感器,充分利用了溶胶-凝胶固定化酶稳定的优点和纳米碳管的高灵敏电催化作用,优化了该酶传感器的制备过程,提高了传感器的电流响应和反应线性.结果表明,sol-gel构建的优化条件是：H2O：TEOS为2.5～3.5,TritonX-100浓度为5%,pH值为5.5.在本实验条件下,多壁纳米碳管的最适固定量为5μl（0.25g/L）,溶胶-凝胶与酶的优化体积比为3：2.工作电位＋0.55V、pH 6.5、25℃为制备传感器的最适工作条件.该传感器对葡萄糖在0.5～6 mmol/L呈线性响应,响应时间为20 s,检出限为0.05mmol/L,45天时的响应值仍保持90%.

英文摘要：

A highly-sensitive glucose biosensor was prepared based on multi-walled carbon nanotubes modified electrode combined with SiO2 sol-gel immobilization method. Its fabrication procedure, related experiment conditions and electrochemical characterization were studied in details. H2O：TEOS = 2.5 - 3.5,5% TritonX-100,pH5.5 were the optimum fabrication procedure parameters of sol-gel. 5μl MWCNTs solution（0.25g/L）, sol-gel/GOD = 3：2（v/v） were the optimum fabrication parameters of enzyme-electrode. The optimum detection potential was ＋ 0.55 V, pH 6.5 at 25 ℃. Under selected conditions the sensor offered a linear range of 0.5 - 6 mmol/L and a detection limit of 0.05 mmol/L. The response time was 20s. The sensor remained 90% of its response after using 45 days.