中文摘要：

应用亲和型生物传感器-Asys生物传感器实时监测不同浓度的肿瘤坏死因子-α（TNF-α）与可溶性肿瘤坏死因子受体-I（sTNFR—I）的亲和反应过程，并应用FASTfit软件对数据进行分析。结果表明：sTNFR—I以2.25ng／mm^2的密度固定于样品池敏感基片表面，TNF-α可与其发生特异性、浓度依赖性亲和反应，应用FASTfit软件进行数据拟合。并计算其反应的动力学常数，其反应符合二级动力学方程，结合速率常数kmaz=4.21×10^4L·mol^-1·s^-1，解离速率常数kdim=2．12×10^-2s^-1，则解离平衡常数kD=5．05×10^-7mol·L^-1，结合平衡常数kA=1．99×10^6L·mol^-1。应用IAsys传感器可实时监测结合和解离反应过程，操作简便、快速，是研究TNF-α和sTNFR相互作用动力学的有效方法。

英文摘要：

The interaction between different concentrations of tumor necrosis factor-α （TNF-α） and soluble tumors necrosis factor receptor-I （sTNFR-I） is studied by using an affinity biosensor-IAsys biosensor, and the data is analyzed by using the FASTfit program, sTNFR-I is immobilized on the cuvette surface with the density of 2.25 ng/mm^2 ,TNF-α then bound to immobilized sTNFR-I specifically in a dose-dependent manner. By FASTfit fitting,the responses fitted the biphasic association quite well, and the error i,s small and random. Kinetic constants for TNF-α binding to sTNFR-I are determined from the plots of kon versus＂ the concentration of TNF-α ： kmax =. 4.21 × 10^4L · mol^-1 · s^-1 ,kdisa =2.12 × 10^-2s^-1 ,kD =5.05 × 10^-7mol · L^-1 ,kA = 1.99 × 10^6L · mol^-1. iAsys biosensor is able to monitor the binding and dissociating courses in real time with simple and quick operation, which provided an effective assay for kinetic analysis of TNF-α/sTNFR-I interaction.