中文摘要：

以多壁纳米碳管（MWCNTs）为电子媒介体和酶的吸附载体，利用层层累积的白组装技术固定葡萄糖氧化酶（GOx）的多层（MWCNTs／GOx）。复合薄膜修饰电极，制备了一种新型葡萄糖生物传感器。结果表明：传感器对葡萄糖的响应电流值随着MWCNTs／GOx复合薄膜层数的不同而变化，当MWCNTs／GOx复合薄膜的层数为6时，响应电流值达到最大。（MWCNTs／GOx）。复合薄膜修饰的葡萄糖生物传感器对3×10^-2moL／L葡萄糖的响应电流为1．63μA，响应时问仅为6.7s。该生物传感器检测的线性范围为5×10^-4～1．5×10^-2moL／L，最低检测浓度可达0．9×10^-4mol／L。

英文摘要：

Take multi-walled carbon nanotubes （MWCNTs） as electronic intermedia and adsorptive carrier of enzyme, a layer-by-layer serf-assemble technique is used to immobilize glucose oxidase （GOx） and form （MWCNTs/GOx） n multilayer films to modify electrode, then a new kind of biosensor is prepared. Results show that the response current to glucose of biosensor is changed with different number of MWCNTs/GOx muhilayer films,and it reaches the maximum value when the number of MWCNTs/GOx layers is 6. The response current of the （MWCNTs/GOx）6 multilayer films-modified biosensor to 3 × 10^-2 mol/L glucose is 1.63 μA while the response time is only 6.7 s. The linear range of this biosensor is 5 × 10^-4 - 1.5 × 10^-2 mol/L and the lowest detectable concentration can even reach at 0.9 × 10^-4 mol/L.