中文摘要：

试图在 vivo 识别 Jinkuishenqi 药片和他们的代谢物的全部的 glycosides (TG ) 的化学部件。一个 HPLC-ESI-MS 方法在老鼠血浆为 TG 的代谢物的分析被开发的方法，在老鼠与 TG 被管理以后。18 混合物全部的结果 A 在 TG，其九是代谢物的口头的管理以后在尿被检测，其它是在 Jinkuishenqi 药片包含的混合物的原来的形式。在他们之中，代谢物是法国的酸， dehydromorroniaglycone， 2-hydroxyacetophenone-4-O-glucuronic 酸酉旨， paeonimetabolin 我， Mlog-2， Mlog-1， 2-hydroxy-4-methoxy acetophenone-5-O-sulfate， 2， 4-dihydroxyacetophenone 和一未知混合物分别地。20 混合物的一个总数在 TG，其八是代谢物的口头的管理以后在排汇物被检测，其它是在 Jinkuishenqi 药片包含的混合物的原来的形式。在他们之中，代谢物是法国的酸， swerosimetabolin A， paeonimetabolin 我， Mlog-2， Mmor-2， Mlog-1 和二未知混合物。用 HPLC-ESI-MS 的结论，在老鼠的 vivo 屏蔽并且分析 TG 的多重混合物和代谢物是可能的。这些代谢物的鉴定和结构说明为 TG 的有效性的进一步的药理学和机制提供了必要数据。

英文摘要：

AIM： To identify the chemical components of the total glycosides （TG） of Jinkuishenqi pills and their metabolites in vivo. METHODS： An HPLC-ESI-MS method was developed for analysis of the metabolites of TG in rat plasma, after the rat was administered with the TG. RESULTS： A total of 18 compounds were detected in urine after oral administration of TG, nine of which are metabolites and the others are the original form of the compounds contained in Jinkuishenqi pills. Among them, the metabolites are gallic acid, dehydromorroniaglycone, 2-hydroxyacetophenone-4-O-glucuronic acid esters, paeonimetabolin I, Mlog-2, Mlog-1, 2-hydroxy-4-methoxy acetophenone-5-O-sulfate, 2, 4-dihydroxyacetophenone and one unknown compound, respectively. A total of 20 compounds were detected in the feces after oral administration of TG, eight of which are metabolites and the others are the original form of the compounds contained in Jinkuishenqi pill. Among them, the metabolites are gallic acid, swerosimetabolin A, paeonime- tabolin I, Mlog-2, Mmor-2, Mlog-1 and two unknown compounds. CONCLUSIONS： Using HPLC-ESI-MS, it was possible to screen and analyze the multiple compounds and metabolites of TG in vivo of rats. The identification and structure elucidation of these metabolites orovided essential data for further oharmacolo~ical and mechanism of effectiveness of TG.