研究施入抗生索溶杆菌菌剂对魔芋软腐病的控制作用,同时采用Biolog方法分析该生防细菌对魔芋根际微生物多样性的影响。田间试验结果显示:施入生防细菌后,抗生索溶杆菌能有效控制魔芋软腐病,还有增产作用,防效可达79.16%,增产14.30%,显著高于水合霉素和对照;还可不同程度地提高魔芋根际微生物Shahnon多样性、均匀度指数和每孔颜色平均变化率(AWCD);并且魔芋根际微生物对碳源的利用情况也发生了改变。与对照相比,施入生防菌剂的处理,对聚合物、胺类、酚类化合物的碳源利用率得以提高,而对糖类、羧酸类、氨基酸类的碳源利用率降低。综合分析认为,施入生防细菌菌剂对魔芋根际微生物群落多样性无明显的负面影响。
The experiments were carried out to investigate the effects of L. antibioticus biocontrol agent on suppressing soft rot of konjac and metabolic functional diversity of konjac rhizosphere microbial community by biolog ECO microplates. The results showed that bio - control agent could suppress the disease and increased yield effectively,bio - control efficacy amount to 79.16% and crop yield increased by 14.30%. In addition to Shannon diversity, Shannon evenness and the average well color development (AWCD) were influenced by bio - control agent. The ability of utilizing carbon source of konjac rhizosphere microbe varied in different treatments. Compared with water control(CK) ,L. antibioticus treatment raised the ability of utilizing polymers, amines and phenolic compounds, but reduced the ability of utilizing carbohydrates, carboxylic acids, amino acids. In conclusion, it is suggested that effects of L. antibioticus agents on the diversity of microbial community diversity of Konjac rhizosphere were not significantly negative.