运用正交试验对石榴多酚的乙醇提取条件进行了优化。考察了影响石榴皮中多酚提取效果的4个因素。结果显示,石榴皮中多酚的最佳提取条件为:20%(体积分数)乙醇作溶剂,料液比(g:mL)1:20,温度50cc,提取时间1h,以该优化条件提取时,多酚粗提物的得率44.98%,纯度50.12%,即石榴多酚得率达到22.86%。
The extraction condition of pomegranate polyphenols was optimized by orthogonal experiment. The results showed that extraction temperature, extraction time, ratio of raw material to solvent and ethanol concentration had significant influences on pomegranate polyphenol extraction, of which the ratio of raw material to solvent exhibited the greatest effect on the extraction yield. The optimum extraction condition was as follows: ratio of pomegranate peel powder to ethanol-water (20 % , volume part) 1 : 20 at 50 ~C for 1 h. Under this condition, the yield of polyphenols from pomegranate peel was 22.86 % while the yield of crude extract and polyphenol content in the extract were 44.98 % and 50.12 %, respectively.