中文摘要：

目的观察Rho激酶抑制剂Y-27632对人气道平滑肌细胞（ASMCs）增殖的影响，为哮喘防治提供新思路。方法组织块贴壁法培养人ASMCs，对数传代后随机分为4组，A组加入含10％FBS的DMEM培养基，B、C、D组分别加入浓度为0．4、2．0、10．0μmol／L的Y-27632＋含10％FBS的DMEM培养基，非放射性细胞增殖（MTS）／吩嗪甲酯硫酸盐（PMS）法检测各组细胞增殖情况（490nm处A值），流式细胞仪分析细胞周期。结果C、D组A值均显著低于A组（P〈0．05、0．01）；C、D组G0／G1期细胞比例显著高于A组，S、G2／M期细胞比例显著低于A组（P〈0．05、0．01）。结论Rho激酶抑制剂Y-27632可抑制人ASMCs增殖，阻滞细胞周期于G0／G1期；本研究为哮喘防治提供了新的选择。

英文摘要：

Objective To investigate the effects of Rho-kinase inhibitor Y-27632 on the proliferation of human airway smooth muscle eeUs （ ASMCs）, so as to provide new ideas for the treatment of asthma. Methods The ASMCs were primarily cultured to log phase by explant method and then divided into four groups, group A were treated with 10% FBS ＋ DMEM, group B , C, D were treated with Y-27632 at the eoncentration of 0.4,2.0,10.0 μmol/L respeetively＋ 10% FBS ＋ DMEM. MTS/PMS assay was used to investigate the proliferation of ASMCs（ A Value at 490 nm）. Flow cytometry was used to ana- lyze the cell eyele. Results The A Value of group C and D were signifieandy lower than that of group A （ P 〈 0.05, 0.01 ）. The cell percentages of G0/G1 phase of group C and D were much higher than that of group A,while cell ratios in S and G2/M were lower than that of group A （P 〈 0.05,0. 01 ）. Conclusions Rho kinase inhibitor Y-27632 earl inhibit the proliferation of ASMCs. This study provides new options for the treatment of asthma.