中文摘要：

嗅觉的 ensheathing 房间(OEC ) 的生存能力上的飞米第二导致激光的损坏的影响被调查。包括细胞的损坏，恢复和死亡的几个 cytokinetic 过程被讨论。用有 100 μW 的力量和 2 μm/s 的切的速度的飞米第二激光，我们与更小的直径把细胞的隆起切了两次成不同地点，然后观察损坏房间的生存能力。在一样的条件下面，有更大的直径的隆起的根被切六次观察细胞的形状的变化。损坏是否最后被定位，有更小的直径的隆起的中间或根，房间生存能力能在 3 h 以内恢复。当损坏与更大的直径位于隆起的根时，损坏房间将以 oncosis 的方法死。包括细胞内部的高 Ca ²⁺ 集中，细胞的词法变化，恢复和 oncosis 的 Cytokinetic 现象被讨论。同时，高 Ca ²⁺ 集中在飞米第二激光外科以后被观察。因此，飞米第二激光外科是为建立房间损坏模型和学习 cytokinetics 的一个重要工具。

英文摘要：

The influence of femtosecond laser-induced damages on viability of olfactory ensheathing cells （OECs）is investigated. Several cytokinetic processes including cellular damage, recovery and death are discussed. Using femtosecond laser with the power of 100μW and cutting speed of 2 μm/s, we cut the cellular protuberance with smaller diameter twice in different locations, and then observe the viability of the damaged cells. Under the same conditions, the root of protuberance with larger diameter is cut six times to observe changes of cellular shape. Whether the damage is located in the end, middle or root of protuberance with smaller diameter, the cell viability can recover within 3h. When the damage is located in the root of protuberance with larger diameter, the damaged cell will die in the way of oncosis. Cytokinetic phenomena including intracellular high Ca^2＋ concentration, cellular morphologic change, recovery and oncosis are discussed. Meanwhile, high Ca^2＋ concentration is observed after femtosecond laser surgery. Therefore, femtosecond laser surgery is an important tool for establishing cell damage model and studying cytokinetics.