中文摘要：

采用化学修饰剂对来源于米曲霉（Aspergillus Oryzae）的外切β-D-氨基葡萄糖苷酶（GlcNase）进行修饰。氯胺-T（Ch-T）和碳二亚胺（EDAC）的修饰结果表明甲硫氨酸残基和羧基是GlcNase的必需基团。用化学修饰剂对GlcNase的羟基、精氨酸残基、酪氨酸残基、巯基和二硫键进行修饰,结果表明：修饰剂在较高的浓度范围内均没有引起酶活的显著降低,因此羟基、精氨酸残基、酪氨酸残基、巯基和二硫键都不是维持GlcNase酶活的必需基团。

英文摘要：

Chemical modification of exo-β-D-glucosaminidase from Aspergillus Oryzae was performed.Results of modification by Ch-T and EDAC indicate that methionine residues and carboxyl groups were essential groups of GlcNase.However,hydroxyl groups,arginine residues,tyrosine residues,sulfydryl groups,and disulfide bonds were not essential groups of GlcNase.