以甲烷磺酸为催化剂和氨基保护剂,苯甲酰氯与壳聚糖酯化合成了O-苯甲酰壳聚糖。产物结构经FITR和1H-NMR光谱分析,酯化反应发生在壳聚糖的羟基,游离氨基得到了很好保护,平均取代度为0.35。以灰霉病菌Botrytis cinerea为受试菌种,研究了O-苯甲酰壳聚糖抑制真菌的活性,结果表明:壳聚糖和O-苯甲酰壳聚糖的有效中质量浓度(EC50)分别为1.55 mg/mL和0.27 mg/mL,O-苯甲酰化壳聚糖的抑真菌活性显著提高。
This study ssing MeSO3H as catalyst and protecting agent to synthesizeO-benzoyl-chitsan by esterification between chitosan and benzoyl chloride.The structure of the product was analyzed by FITR and 1H-NMR spectroscopy.The esterification mainly occurred on OH group rather than-NH2 group of chitosan.The average degree of substitution(DS) was 0.35.The anti-fungi activity of O-benzoyl-chitsan against the grey mould,Botrytis cinerea was carried out.The result showed that the EC50 of chitosan and O-benzoyl-chitosan were 1.55 mg mL-1 and 0.27 mg mL-1,respectively.The anti-fungi activity of O-benzoyl-chitosan was much stronger than that of chitosan.