中文摘要：

目的：在成功建立目前公认的老年性痴呆（AD）体外模型基础上，观察补肾化痰益智法抗炎治疗AD的作用及机制。方法：运用Aβ1-42作用于BV-2细胞，同时用补肾化痰益智方含药血清进行干预，以吲哚美辛为对照。ELISA法检测细胞释放白介素-1β（IL—1β）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF—α）的情况，RT—PCR法检测IL-1β mRNA的表达。结果：补肾化痰益智组与吲哚美辛组比较，能够更有效抑制IL-1β的表达（P〈0．05），并能够从基因水平调节IL-1β mRNA的表达，从而抑制BV-2细胞分泌炎性因子的作用。结论：AB神经毒性与AD免疫炎性反应具有相关性。补肾化痰益智法能够调控炎性细胞因子IL-1β、IL-6、TNF—d及IL-1β mRNA的表达，从基因水平抑制炎性反应。

英文摘要：

Objectives： Based on the successful establishment in vitro model of Alzheimer＇s disease （AD）, the effects and mechanism on anti-inflammatory of AD was investigated by Bushen Human Yizhi Recipe （BSHTYZR）. Methods： BV-2 cells effected by Aβ1-42 were produced for microglia model of AD, and then intervened by the serum containing-BSHTYZR, which was compared to the serum containing-Indometacin Enteric-Tablets （Indometacin）. The protein expression of IL-1β, IL-6, TNF-α was examined by ELISA, and IL-1β mRNA was detected by RT-PCR. Results： The BSHTYZ group could more effectively inhibit the expression of IL-1β （P〈0.05）. BSHTYZ Method could regulate the expression of IL-1β mRNA from the level of gene, then to suppress the secretion of inflammatory factors on BV-2 cells. Conclusion： There is a relationship between Aβ neurotoxicity and immune inflammatory response in AD. BSHTYZ method can regulate inflammatory cytokine IL-1β, IL-6, TNF-u and IL-1β mRNA＇s expression, and inhibit the inflammatory response in AD from the genetic level.