中文摘要：

目的探索解偶联蛋白1（UCP1）基因启动子甲基化水平与肥胖的关系。方法选取某医院腹部手术患者116例,超重/肥胖组50例（BMI≥24.0）,正常体重组66例（18.5≤BMI〈24）,提取两组白细胞DNA并进行甲基化处理,采用质谱法定量检测UCP1基因甲基化水平,比较两组甲基化差异及各CpG位点的甲基化水平与BMI的关系。结果 t检验结果显示,正常体重组和超重/肥胖组的各CpG位点的差异无统计学意义（P〉0.05）;多重线性回归分析发现,位点UCP1-2_CpG_10.11.12.13与BMI存在线性关系（回归系数为15.370,P〈0.05）。结论 UCP1基因甲基化可能与肥胖存在一定关系。

英文摘要：

Objective To observe the relationship between the methylation status in promoter region of uncoupling protein 1（ UCP1） gene and obesity. Methods A casecontrol study based on the hospital consisted 116 people was carried out,according to the body mass index（ BMI）,the subjects were divided into two groups,the overweight and obesity group with 50 samples（ BMI≥24. 0） and the normal weight group with 66 samples（ 18. 5≤BMI 24）,DNA samples were extracted from white blood cell and treated by hydrogen sulfite. Then mass spectrometry method was used to quantificationally detect the methylation level UCP1 gene promoter. The difference between the two groups was compared and the relationships between CpG sites and BMI were explored. Results Statistical analysis showed that the methylation differences between normal weight and overweight or obese group were not statistically significant,however,the CpG site UCP1-2_ Cp G_ 10. 11. 12. 13 had statistical significance in correlation coefficients with BMI according to multiple linear regression method（ regression coefficient was 15. 370,P 0. 05）. Conclusion The UCP1 gene promoter methylation may be a factor for adult obesity.