中文摘要：

采用PCR方法从鳜鱼（Siniperca chuasti）基因组DNA中分离得到了鳜鱼肌球蛋白轻链2基因启动子序列,其大小为890 bp,包含1个TATA框、3个MEF2结合位点和5个与b HLH转录因子相结合的E-框（CANNTG）.用同样的方法从鲢鱼基因组DNA中分离得到鲢鱼肌球蛋白轻链2基因启动子序列,其大小为870bp,包含了1个TATA框、1个MEF2结合位点和3个与bHLH转录因子相结合的E-框.鳜鱼比鲢鱼肌球蛋白轻链2基因启动子转录调控元件更加丰富.

英文摘要：

The myosin light chain 2 promoter was isolated from Siniperca chuasti genomic DNA by polymerase chain reaction（PCR） and its contains 890 base pairs,including 1 TATA box,3 MEF2 binding sites and 5 E-box（CANNTG）,where the bHLH transcription factors were combined.The Mylopharyngodon piceus myosin light chain 2 promoter was also isolated from its genomic DNA by polymerase chain reaction（PCR）,containing 870 base pairs,including 1 TATA box,1 MEF2 binding site and 3 E-box（CANNTG） where the bHLH transcription factors were combined.Distance of start sites of the proximal MEF2 binding sites and TATA-box of MLC2F promoter was 28 bp and 21 bp in the Siniperca chuasti and the Mylopharyngodon piceus,respectively.The comparative analysis of MLC2 promoters of the two fish species revealed the key regulatory domain for gene expression and provided us better understanding of MLC2 functions in fish.