中文摘要：

肌浆球蛋白重链(MyHC ) 是肌肉的主要结构、收缩的蛋白质之一。我们孤立编码草鲤鱼的 MyHC 的 cDNA 克隆， Ctenopharyngodon idella。顺序包括 5 934 bp，包括 5 814 bp 编码 1 937 残余的一个氨基酸序列的开的读框架。推出的氨基酸顺序显示出 69% 相同到兔子快骨胳的 MyHC 和 73% 鈥 ?6% 相同到从官员鱼，白眼绿鳕，白 croaker，鱼饵大马哈鱼，和鲤鱼的 MyHCs。subfragment-1 和轻 meromyosin 区域的通常认为的序列显示出 61.4% 鈥 ?0% 相同到另外的鱼 MyHCs 的相应区域。MyHC 基因的织物特定、发展的阶段特定的表情被量的即时 PCR 分析。MyHC 基因与肾，怒气和肠相比在肌肉显示出最高的表示。发展地，从神经形成舞台的 MyHC mRNA 表示有渐渐的增加到尾巴芽舞台。最高的表示在孵化被检测幼虫。我们从草鲤鱼的 MyHC 基因上的工作为鱼提供了有用信息分子的生物学和鱼 genomics。关键词草鲤鱼 - 即时 PCR - 肌浆球蛋白重链 - 快骨胳的肌肉 - 中国的国家自然科学基础支持的基因表示(Nos. 30972263， 30771644 ) ，并且 CHU Wuying 和 FU Guihong 同等地贡献了到这份报纸的湖南省(号码 09jj6037 ) 的自然科学基础

英文摘要：

The myosin heavy chain （MyHC） is one of the major structural and contracting proteins of muscle. We have isolated the cDNA clone encoding MyHC of the grass carp, Ctenopharyngodon idella. The sequence comprises 5 934 bp, including a 5 814 bp open reading frame encoding an amino acid sequence of 1 937 residues. The deduced amino acid sequence showed 69% homology to rabbit fast skeletal MyHC and 73%-76% homology to the MyHCs from the mandarin fish, walleye pollack, white croaker, chum salmon, and carp. The putative sequences of subfragment-1 and the light meromyosin region showed 61.4%-80% homology to the corresponding regions of other fish MyHCs. The tissue-specific and developmental stage-specific expressions of the MyHC gene were analyzed by quantitative real-time PCR. The MyHC gene showed the highest expression in the muscles compared with the kidney, spleen and intestine. Developmentally, there was a gradual increase in MyHC mRNA expression from the neural formation stage to the tail bud stage. The highest expression was detected in hatching larva. Our work on the MyHC gene from the grass carp has provided useful information for fish molecular biology and fish genomics.