采用抑制消减杂交(SSH)方法构建了对吡虫啉敏感和抗吡虫啉褐色虱种群的正反向差减cDNA文库,克隆了受吡虫啉调控的相关基因。结果表明,筛选到的120个刚性克隆代表92个互不重复的单丛因,其中17个与动物的已知蛋白基因存在较高的同源性。筛选剑的单基因,分别涉及信号传导、生物及非生物胁迫、生殖等。
The forward and reverse subtracted cDNA libraries of imidacloprid resistant and imidacloprid-susceptible brown planthopper (Nilaparvata lugens Still) populations were constructed by suppression subtractive hybridization (SSH), and lhe relative genes regulated by imidacloprid were cloned. The results showed that 120 positive clones nblained represented 92 different single genes, in which 17 shared higher homology with known prolein genes of animals in the GenBank. These single genes involved signal transduction, biotic and abiotic slress, reproduction, and etc.