中文摘要：

目的：探索PKC信号通路在血管内皮生长因子（VEGF）介导的骨髓源间充质干细胞（MSC）迁移中的作用及其机制。方法：以P3MSC为实验材料,利用Tanswell体外迁移体系,观察不同浓度的VEGF对MSC迁移的影响,在Straurosporine、G06976、Pseudo Z等信号转导途径阻断剂的干预下观察VEGF对迁移的影响。结果：MSC体外迁移能力随VEGF浓度（0.1,1,10,20,50 ng/mL）的递增而逐渐增强,20 ng/mL浓度时,MSC迁移到滤膜上的细胞数接近于峰值;Straurosporine、G06976、Pseudo Z对MSC迁移均有影响,其中Straurosporine、Pseudo Z对MSC迁移阻断的效应最显著。结论：VEGF所介导的MSC迁移与蛋白激酶C信号途径有关,且PKC-ζ途径可能处于中心环节。

英文摘要：

Objective To explore the role of PKC signal pathway in the migration of bone marrow-derived mesenchymal stem cells（MSCs） mediated with VEGF and its possible mechanism.Methods Transwell in vitro migration assay system with P3MSC medium were taken to evaluate the effect of different concentration VEGF on the migration of MSC.The effect changes of VEGF on the migration were observed after giving signal transduction inhibitor,such as Straurosporine,G06976,Pseudo Z.Results The migration of MSC was in a dose-dependent manner and increased with the increasing concentration of VEGF,and reached the peak at 20 ng/mL.Straurosporine,G06976,Pseudo Z all could affect the migration of MSC,and the effect of Straurosporine,Pseudo Z were more obviously.Conclusion The migration of MSCs mediated with VEGF was related to PKC signal pathway,and the PKC-ζ signal pathway may be a key link in MSCs migration.