中文摘要：

目的：利用Boyden chamber体外迁移体系以及活体状态下观察基质细胞衍生因子-1（SDF-1）对间充质干细胞（MSC）迁移的影响。方法：利用Boyden chamber体外迁移体系，先观察不同浓度hSDF-1对MSC迁移的影响，随后利用活体迁移体系，观察MSC转染Ad—hSDF-1后迁移的变化。最后利用上述体系经不同的阻断剂分别处理MSC，观察其对MSC迁移的影响。结果：MSC体外迁移能力随着hSDF-1α浓度递增而逐渐增强，并且Wortmannin、LY294002、PD98059、SB203580、PKC-ζ假底物、U70312、AMD3100、Verapamil对MSC迁移均有影响，其中PKC-ζ假底物、U73122、AMD3100对MSC迁移阻断的效应最显著。结论：SDF-1／CXCR4所介导的MSC迁移与PI3K、MAPK、PI-PLC／PKC等信号途径有关，且PKC途径可能处于中心环节。

英文摘要：

Objective： To explore the signal transduction mechanism of stromal derived factor-1 （SDF-1） on migration of mesenchymal stem cell （MSC）. Methods： The SDF-1 density-dependent of MSC migration was observed. And the characteristics of MSC migration were observed for 1, 2 and 3 days after Ad- EGFP-hSDF-1α （100μl） was added into1 single-disposition MSC. Subsequently, 12 hours later MSC transfected by Ad-EGFP-hSDF-1α were treated with 50 nmol wortmannin, 10μmol/L LY294002, 50μmol/L PD98059, 10μmol/L, U73122 and 126μmol/L AMD3100 and 50 nmol/L verapamil, respectively. Results：The efficiency of MSC migration increased gradually with the density increasing of hSDF-1, and after MSCs treatment with 50 nmol wortmannin, 10μmol LY294002,50μmol PD98059,30μmol SB203580,50μmol PKC-PI 10μmol U73122 and 126μmol/L AMD3100, respectively, the ability of MSC migration was decreased. Importantly, the ability of MSCs migration obviously decreased when MSCs with U73122, AMD3100 treatment by different migration assay systems. Conclusion：SDF-1/CXCR4-mediated MSCs migration is related with mitogen-activated protein kinase （MAPK）,phosphatidylinositol phospholipase C（PI-PLC） and protein kinase （PKC） signal pathways, PKC signal pathway may be the central role for MSCs migration.