中文摘要：

目的通过研究阳和平喘颗粒对哮喘大鼠肺组织水通道蛋白1（AQP1）和水通道蛋白5（AQP5）表达的影响,旨在从水液代谢及气道黏液分泌的角度探讨阳和平喘颗粒治疗支气管哮喘的作用机制。方法 SD大鼠90只随机分为正常组（N）、模型组（M）、阳和平喘颗粒低、中、高剂量治疗组（YHPC-L、YHPC-M和YHPC-H）和地塞米松治疗组（DXM）,每组15只。其中,M、YHPC-L、YHPC-M、YHPC-H和DXM组采用卵蛋白（OVA）腹腔注射致敏及雾化吸入的方法建立哮喘大鼠模型,同时应用氢化可的松腹腔注射复制肾阳虚模型,N组则均使用生理盐水作为对照。采取HE染色检测各组大鼠肺组织形态学改变,应用Real-time PCR和Western blot检测各组大鼠肺组织AQP1和AQP5mRNA及蛋白表达水平的变化,采用免疫组化法检测AQP1和AQP5在肺组织的分布及表达水平变化。结果与N组相比,M组AQP1和AQP5在mRNA及蛋白表达水平均发生显著下降（P〈0.01）;与M组相比,YHPC-L、YHPC-M和YHPC-H组AQP1和AQP5的表达水平均显著上升（P〈0.05～0.01）,且具有一定的剂量依赖效应;与DXM相比,YHPC-L对AQP1和AQP5的作用效果低于DXM（P〈0.05）,而YHPC-M和YHPC-H则与之具有相当的作用效果（P〉0.05）。结论阳和平喘颗粒可通过上调AQP1和AQP5的表达而调控水液代谢平衡及气道黏液分泌,进而发挥其治疗支气管哮喘的作用。

英文摘要：

OBJECTIVE To investigate the effects of Yanghe Pingchuan Granules on the expression of AQP1 and AQP5and explore the mechanism of Yanghe Pingchuan Granules in treating asthma from regulating water metabolism and airway mucus secretion.METHODS 90 SD rats were randomly divided into six groups which were the normal group（N）,asthma model group（M）,low,medium and high-dose of Yanghe Pingchuan groups（YHPC-L,YHPC-M and YHPC-H）and Dexamethasone group（DXM）.The M,YHPC-L,YHPC-M,YHPC-H and DXM groups were sensitized with OVA by intraperitoneal injection and inhalation,then Hydrocortisone was used to replicate kidney-yang deficiency model,while the N group was treated with saline as control.HE staining was used to observe the pathological changes in lung tissues.Real-time PCR and Western blot was applied to detect the mRNA and protein expression of AQP1 and AQP5in lung tissue,respectively.Immunohistochemical was applied to examine the AQP1 and AQP5distribution and expression in lung tissue.RESULTS The mRNA and protein expression of AQP1 and AQP5in M group was significantly lower than in N group（P〈0.01）.Compared with M group,the expression of AQP1 and AQP5increased highly after treated with low,medium and high-dose of Yanghe Pingchuan Granules（P〈0.05～0.01）with dose-dependent effect.Meanwhile,the effect of low-dose Yanghe Pingchuan Granules was lower than DXM（P〈0.05）,but the medium and high-dose of Yanghe Pingchuan Granules had a considerable effect（P〉0.05）.CONCLUSION Yanghe Pingchuan Granules could maintain water homeostasis and regulate airway mucus secretion by up-regulating the expression of AQP1 and AQP5.