中文摘要：

射频热凝毁损术治疗帕金森病有效率达90%以上,但手术过程中毁损体积的量化问题研究得不多,通过控制毁损温度和时间来确定毁损灶的范围是有效的,但其量化关系还未见报道。本研究建立了体外蛋清射频毁损灶和活体内大鼠脑射频毁损灶模型,探讨毁损体积随毁损温度及时间两个参数变化的规律,利用1stOpt数学拟合工具拟合了相关度较高的数学模型,通过这一关系就能够根据预毁损体积选择相应的温度和时间,提高毁损术效果;使用特制的内置四光纤探头测量了大鼠脑靶点处的生物组织光学参数优化散射系数μs’。实验数据表明,μs＇的变化规律与相应毁损温度时间下的毁损体积变化规律相似,证明了其与射频毁损灶体积有很好的相关性。建立了毁损体积与优化散射系数之间的关系模型,为探索近红外光谱技术在射频毁损手术实时监控中的应用提供了实验依据。病理学检测证明了实验的有效性。研究结果为探索近红外光谱技术在帕金森病射频毁损手术实时监控中的可行性提供了实验依据。

英文摘要：

The efficiency of Rf thermal damage to coagulate surgical treatment for Parkinson＇s disease is over 90%,but during the operation the quantification study of damage volume is rare,controlling the damaged temperature and time to determine the scope of damage is effective,but its quantitative relation is still not seen in reports.This study established a large number of radiofrequency ablation volume models which include in vivo fresh albumen and in vitro brain of normal adult SD rats.Based on the statistical analysis of in vivo and in vitro volume,we obtained the regression equation between the lesion temperature,lesion time and the lesion volume and calculated the optimal lesion time and lesion temperature to achieve precise prediction ability for the clinical application.The micro-probe of near infrared spectroscopy（NIRS） was used to record the changes of optical parameters（μs＇） during the lesion process and explored the correlation between the RF ablation volume and the μs＇.It will provide sound foundation for the application of NIRS in the areas of real-time monitoring of surgical RF ablation.The pathology tests include MRI,light microscopy and electron microscopy proved the experiment data＇s effectiveness.