中文摘要：

目的：探讨MPTP小鼠帕金森病（PD）模型脑内半胱氨酰白三烯受体CysLT1R、CysLT2R和GPR17表达分布特征。方法：C57BL／6小鼠连续5d皮下注射MPTP25mg／kg诱导PD模型，第10d处死动物。应用免疫组化方法，观察CysLT1R、CysLT2R和GPR17在黑质表达的变化，并用免疫双标方法进行细胞定位。结果：正常小鼠黑质中有CysLT1R、CysLT2R和GPR17表达，主要表达在TH阳性多巴胺能神经元以及小胶质细胞，CysLT2R还表达于星形胶质细胞；其中，在多巴胺能神经元中91％表达GPR17，77％表达CysLT1R，52％表达CysLT2R。与对照组比较，PD模型组小鼠黑质中TH阳性细胞数显著减少；同时CysLT1R、CysLT，R和GPR17阳性细胞数以及表达CysLT1R、CysLT2R和GPR17的多巴胺细胞数均显著减少。在正常小鼠纹状体中有CysLT1R、GPR17表达，主要表达于神经元；而CysLT2R表达很少，主要表达于星形胶质细胞。PD模型组小鼠纹状体中CysLT1R、GPR17阳性细胞数减少；但CysLT2R表达升高，主要表达于增生的星形胶质细胞。结论：CysLT1R、CysLT2R和GPR17可能参与MPTP所致的小鼠PD损伤过程。

英文摘要：

Objective ： To examine the spatiotemporal profiles and localization of CysLTj R, CysLT2 R and GPR17 in mice with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine （MPTP）-indueed Parkinson disease （PD）. Methods： PD model was induced by subcutaneous injection of MPTP （25 mg/kg） for 5 d in adult male C57BL/6 mice. At d10 after MPTP injection, the expression and cellular localization of CysLTIR, CysLT2 R and GPR17 in the substantia nigra were detected by immunohistochemistry and immunofluorescence. Results： CysLT1 R, CysLT2R and GPR17 were normally localized in TH-positive dopaminergic neurons and microglia, while CysLT2 R was also expressed in astrocytes. In dopaminergic neurons,approximately 91% co-expressed GPR17,77% co-expressed CysLT1R and 52% co-expressed CysLT2R. Compared with the control group, TH-positive cells in the substantia nigra were significantly reduced in PD mice. CysLTt R, CysLT2 R and GPR17-positive cells were significantly reduced; and CysLT1 R, CysLT2 R, GPR17-positive dopaminergic neurons were also significantly reduced in the PD group. In the striatum,both CysLT1R and GPR17 were normally expressed in neurons; whereas CysLT2R was expressed in astrocytes. In PD striatum, CysLT1 R and GPR17-positive cells were decreased, but CysLTiR expression was significantly increased which mainly expressed in the proliferating astrocytes. Conclusion： CysLT1 R,CysLT2Rand GPR17 may be involved in the MPTP-induced PD damage in mice.