中文摘要：

目的：建立同时测定中药远志中有效成分远志酮Ⅲ和3,6’-二芥子酰基蔗糖的HPLC含量测定方法。方法：采用Kromasil C18（250mm×4.6mm,5μm）色谱柱,以乙腈-0.05%磷酸水溶液（18：82）为流动相,流速1.0mL.min-1,检测波长320nm,柱温30℃。结果：远志酮Ⅲ及3,6’-二芥子酰基蔗糖的线性范围分别为0.5～4.0μg（r=0.9996）和0.5～8.0μg（r=0.9996）,其平均回收率（n=6）分别为97.1%（RSD=0.87%）和99.4%（RSD=1.5%）。结论：该方法准确,重复性好,能够同时对远志中2种有效成分进行含量测定,为远志药材的质量控制提供了有效的方法。

英文摘要：

Objective：To develop an HPLC method for simultaneous determination of the two active ingredients,named polygalaxanthone Ⅲ and 3,6’-disinapoyl sucrose in the roots of Polygala tenuifolia.Methods：The marker compounds of polygalaxanthone Ⅲ and 3,6’-disinapoyl sucrose were separated on a Kromasil C18 column（250 mm×4.6 mm,5 μm）,the mobile phase was acetonitrile-0.05% phosphoric acid aqueous solution（18：82）at a flow rate of 1.0 mL·min-1,the detection wavelength was set at 320 nm and the column temperature was 30 ℃.Results：The assay was linear in the ranges of 0.5-4.0 μg（r=0.9996）for polygalaxanthone Ⅲ and 0.5-8.0 μg（r=0.9996）for 3,6’-disinapoyl sucrose.The average sample recoveries（n=6）were 97.1%（RSD=0.87%）and 99.4%（RSD=1.5%）for these two constituents,respectively.Conclusion：This accurate and reproducible method realizes the simultaneous determination of the two components in P.tenuifolia,which supplies an effective method for the quality control of P.tenuifolia.