中文摘要：

目的对影响化合物3,6′-二芥子酰基蔗糖稳定性的因素进行考察,寻找能够使其稳定存在的条件,为该化合物今后进一步研究及开发提供参考。方法采用紫外分光光度法（UV）和高效液相色谱法（HPLC）,考察3,6′-二芥子酰基蔗糖在不同溶剂、不同pH、不同温度和光照条件下的稳定性,并采用高效液相-二极管阵列检测-质谱（HPLC-DAD-MS）联用技术对光照以及365 nm紫外灯下的转化产物进行分析。结果UV和HPLC具有较好的相关性,二者的分析结果均表明溶剂的种类、pH值、温度和光照条件对3,6′-二芥子酰基蔗糖稳定性具有一定影响。转化产物的紫外和质谱数据分析结果表明,3,6′-二芥子酰基蔗糖光照及紫外灯下照射转化的主要产物是芥子酰基双键顺反异构化的产物。结论UV可用于3,6′-二芥子酰基蔗糖稳定性的快速分析。在该化合物的分离纯化及其分析检测时,应采用醇为溶剂,在中性或酸性条件下,低温、避光操作。

英文摘要：

OBJECTIVE To investigate the factors influencing the stability of 3,6′-disinapoyl sucrose,and to clarify the suitable storage method for its further research and development.METHODS UV and HPLC methods were used to detect the stability of 3,6′-disinapoyl sucrose in different solvents,temperatures,pH values,and illuminations.HPLC-DAD-MS was used to analyze the transformed products of 3,6′-disinapoyl sucrose after illuminating under the sunshine and the UV lamp of 365 nm.RESULTS There was good correlation between the results of UV and HPLC methods.The results showed that the stability of 3,6′-disinapoyl sucrose was influenced by the solvent,pH value,temperature and illumination.The analysis result of the transformed products by UV and MS indicated that the isomers of cis and trans-double bond of the sinapoyl group were the main transformed products of 3,6′-disinapoyl sucrose after illumination.CONCLUSION UV method could be used for the rapid analysis of the stability of 3,6′-disinapoyl sucrose.During the separation,purification and analytical process of this compound,it prefer to a neutral or an acid alcoholic solvent in a low temperature,and to keep away from the light.