中文摘要：

目的研究大川芎方（川芎、天麻）效应组分在硝酸甘油致实验性偏头痛模型大鼠血内、脑内移行成分归属，追溯其从体外-血浆-脑脊液-脑组织的移行趋势。方法以HPLC-DAD为研究手段，建立大川芎方效应组分体内外指纹图谱，并与9个对照品进行成分确认。结果确定大川芎方效应组分中源自于川芎的成分追溯为：体外（20个）-血浆（9个）-脑脊液（6个）-脑组织（5个）；确认的入脑成分为阿魏酸、洋川芎内酯I、洋川芎内酯H；源自于天麻的成分追溯为：体外（21个）-血浆（4个）-脑脊液（3个）-脑组织（1个），确认的人脑成分为天麻素、天麻苷元。结论该结果可确立大川芎方的入脑成分，为准确阐明大川芎方治疗偏头痛的药效物质基础提供依据。

英文摘要：

AIM To study the attribution of characteristic effective ingredients of Dachuanxiong Decoction （ Li- gustici chuangxiong rhizome , Gastrodiae rhizome） （DCXD , and trace back the migratory pathways from in vitro- plasma-cerebrospinal fluid-brain tissue in experimental rats migraine model induced by nitroglycerin. METHODS The HPLC-DAD was used as the analytic methed to establish in vitro and in vivo fingerprint chromatograms of DCXD, and nine constituents were confirmed. RESULTS The in vitro （20 constituents） -plasma （9） -cerebro- spinal fluid （6） -brain tissue （5） could date back to Ligustici chuangxiong rhizome and had been verified that the ingredients absorbed into brain were ferulic acid, senkyunolide I and senkyunolide H （C15）. Meanwhile in vitro （21 constituents） -plasma （4） -cerebrospinal fluid （3） -brain tissue （1） could date back to Gastradine rhizome. And it was confirmed that the ingredients brought into the brain were gastrodin and 4-hydroxyphenethyl alcohol. CONCLUSION The findings could provide the useful information for the determination of bioactive substances of DCXD which is used to treating migraine.