中文摘要：

目的：建立测定小鼠血浆中洋川芎内酯Ⅰ浓度的高效液相色谱方法,并应用于药动学研究。方法：小鼠单剂量静注或灌胃给予（104 mg·kg-1）洋川芎内酯Ⅰ,在给药后不同时间取血,血浆经沉降蛋白处理后,采用HPLC法测定洋川芎内酯Ⅰ的浓度,使用Megres-C18色谱柱（150 mm×4.6 mm,5μm）,以乙腈-2.0%冰醋酸（1∶3,v/v）为流动相,流速为1.0 mL·min-1,柱温为30℃,洋川芎内酯H为内标,检测波长278 nm。结果：在0.075～30 mg·L-1范围内,洋川芎内酯Ⅰ在血浆中线性关系良好,定量下限为0.075 mg·L-1,方法回收率大于85%,日内日间RSD均小于5%,样品在室温下放置24 h,-4℃放置5 d,经过3次冻融循环后基本稳定。小鼠静注给药后主要药代动力学参数分别为t1/231.93 min,AUC（0-∞）2738.80 mg·min·L-1,MRT（0-∞）31.61 min;小鼠灌胃给药后主要药代动力学参数分别为t1/240.07 min,AUC（0-∞）892.42 mg·min·L-1,MRT（0-∞）61.38 min。经计算,洋川芎内酯Ⅰ在小鼠体内的绝对生物利用度为32.19%。结论：所建立的方法可靠,简便快速,适用于洋川芎内酯Ⅰ小鼠体内血药浓度测定及药代动力学的研究。

英文摘要：

Objective：To establish an HPLC method for the determination concentration of senkyunolide I in mice plasma for pharmacokinetic study. Methods： The mice were intravenously administrated of a single dose or intragas- tricly administrated senkyunolide I（ 104 mg · kg-1）. The plasma samples after protein settlement processing were analyzed for the senkyunolide I concentration by HPLC. The Megres - C18 （150 mm× 4. 6 mm,5 μm）column was adopted,the mobile phase was acetonitrile-0.2% glacial acetic acid（ 1：3 ,v/v）at a flow rate of 1.0 mL · min-1 under 30 ℃, the senkyunolide H was marked as the internal standard, and the detection wavelength was set at 278 nm. Results： Excellent liner relationship was obtained in the range of 0.075 to 30 mg · L - 1, the limit determination of senkyunolide I was 0.075 mg · L -1. The recovery of the method was more than 85% ,and the intra - and inter - day RSDs were less than 5 %. The samples were stable for 24 h at room temperature ,5 days at -4 ℃, and remained stable after three freeze -thaw cycles. The pharmacokinetic parameters were as follows： t1/2 31.93 rain, AUC（0-∞） 2738.80 mg. min. L-1,MRT（0-∞） 31. 61 rain in mice with intravenous administration, and t1/2 40. 07 min, AUC （0-∞ ） 892.42 mg · min · L-1, MRT（0-∞ ） 61.38 min in mice with intragastric administration. The absolute bio- availability of senkyunolide I in mice was calculated to be 32.19%. Conclusion： The method is reliable, simple, rapid, and suitable for the pharmacokinetic study and determination of senkyunolide I in plasma.