中文摘要：

应用HPLC法建立四逆汤中甘草的特征图谱，研究配伍对四逆汤中甘草特征峰的影响。采用PhenomenexGeminiC18色谱柱（250i／lrn×4．6mrn，5μm），流动相为乙腈-0．05％Z氟乙酸水溶液，梯度洗脱，流速0．8mL-min，检测波长232nm，柱温30℃。该方法稳定、可靠、精密度好，可用于四逆汤中甘草特征图谱的研究；以甘草苷为参比峰，标定了四逆汤中的20个特征峰，其中6个特征峰来源于甘草，6个特征峰共同来源于甘草和附子干姜汤：与单味甘草相比，全方中甘草特征峰峰面积均有不同程度的降低。配伍对四逆汤中甘草特征峰有较为显著的影响，使甘草中的甘草苷等特征成分溶出量降低。

英文摘要：

The paper is to report the establishment of an HPLC specific chromatogram of Glycyrrhiza in Sini decoctions and the influence of combination on the specific chromatogram. The RP-HPLC method was used with a Phenomenex Gemini C18 column （250 mm x 4.6 mm ID, 5μm）, and acetonitrile-0.05% trifluoroacetic acid （gradient elution） as mobile phase. Flow rate was 0.8 mL＇min-1 and the detection wavelength was set at 232 nm. The temperature of column was 30 °C. The method is stable and reliable with a good reproducibility, it can be used to determine the specific chromatogram of Glycyrrhiza in Sini Decoctions. Twenty peaks were selected as specific peaks in Sini Decoction with liquiritin peak as the reference peak. Six of them were from Glycyrrhiza and the other 6 peaks were from both Glycyrrhiza and Ganjiangfuzi Decoction. The areas of specific peaks of Sini Decoctions were smaller than those in the chromatogram of Glycyrrhiza. The specific chromatogram of Glycyrrhiza in Sini Decoctions is markedly influenced by Radix Aconiti Carmichaeli and Rhizoma Zingiberis. The areas of the specific peaks in Sini Decoctions were reduced obviously. The method is stable and reliable with a good reproducibility, it can be used to determine the specific chromatogram of Glycyrrhiza in Sini Decoctions.