应用Affymetrix基因芯片研究了4 kJ/m2短波紫外线(UV-C)照射后7 d番茄总基因表达谱的变化情况。结果表明,UV-C照射后7 d,5 177个基因在杂交后发生变化,差异表达变化倍数值在大于2和小于-2范围内基因共481个,其中上调基因331个,下调基因150个。上调基因主要包括细胞壁相关基因、信号转导基因、胁迫相关基因和抗性基因等;下调基因主要包括乙烯相关基因和初级代谢基因等。研究结果为探索采后UV-C照射诱导番茄抗性反应、抑制乙烯合成,从而推迟成熟、延长番茄采后寿命的分子机制提供了基础。
To obtain an overall view on transcript modification during the tomato fruit in response to postharvest UV-C irradiation(4 kJ/m2),a microarrary analysis was performed by using Affymetrix tomato genechip arrays.The results showed that 331 and 150 genes were up-or down-regulated,respectively,more than two folds in postharvest UV-C irradiated tomato fruit as compared with that in control fruit.The up-regulated genes mainly involved cell wall related,signal transduction,stress related and defense response.Conversely,genes related to ethylene and primary metabolism were generally down-regulated.These results opened ways to probe into the molecular mechanisms of the effects of postharvest UV-C irradiation on increasing disease resistance,delaying ripening and prolonging postharvest life in tomato fruit.