中文摘要：

为了研究1,2,4-三氯苯（1,2,4-TCB）对斑马鱼（Danio retio）肝细胞DNA的损伤作用,采用不同浓度的1,2,4-TCB溶液（0、1、5、10 mg/L）对体外培养肝细胞进行染毒,应用单细胞凝胶电泳（彗星实验）检测肝细胞DNA的损伤效应。结果发现,染毒2 h后,与对照组相比,当1,2,4-TCB浓度大于5 mg/L时,各染毒组细胞尾长、尾部DNA百分率均极显著升高,即1,2,4-TCB染毒引起了肝细胞DNA的严重断裂;随着1,2,4-TCB浓度的增加,DNA损伤程度加剧,细胞尾长、尾部DNA百分率及尾距与1,2,4-TCB染毒浓度呈明显的剂量-效应关系。结果表明,水体中残留的1,2,4-TCB可导致斑马鱼肝细胞DNA损伤。

英文摘要：

In order to explore the DNA damage in cells induced by 1, 2, 4-TCB, hepatocyte cells of zebrafish cultured in vitro were exposed to different concentrations of 1, 2, 4-TCB （0, 1, 5, 10 rag/L） for 2 h. Then, the comet assay was applied to measure the DNA damage. Results showed that tail length, tall DNA rate of hepatocyte cells both significantly increased when treated with more than 5 mg/L 1, 2, 4-TCB compared with the control group. Meanwhile, the DNA damage increased gradually with the increase of 1, 2, 4-TCB concentration. There was an obvious dose relationship between 1, 2, 4-TCB concentration and DNA damage （tail length, tail DNA rate, tail moment）. It was concluded that 1, 2, 4-TCB could induce DNA damage in hepatocyte cells of zebrafish.