中文摘要：

实验室条件下，通过1，2，4三氯苯（1，2，4-TCB）染毒对成年斑马鱼及幼鱼体内超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和碱性磷酸酶（AKP）活性进行测定和比较，从而确定1，2，4-TCB暴露对斑马鱼的毒性机制。结果发现，低质量浓度（2、4、8mg／L）1，2，4-TCB处理对SOD活性有一定的应激作用，且在第8天明显高于对照组；16mg／L1，2，4-TCB对SOD活性明显抑制。4～10d时4mg／I玻E理组CAT活性与对照组呈显著差异，2、4、8mg／L处理组CAT活性随时间延长先升高后降低。各处理组AKP活性在前4d基本无显著变化，2、4mg／L处理组AKP活性在第8天与对照组呈极显著差异；16mg／L处理组AKP活性低于对照组。斑马鱼胚胎暴露1，2，4-TCB孵化第6天后，3．46mg／L处理组幼鱼蛋白质与对照组呈显著差异，其他处理组与对照组无显著差异。各处理组幼鱼体内s（）D、CAT和AKP活性与对照组基本无显著变化。幼鱼比成年斑马鱼对1，2，4-TCB影响更加敏感。

英文摘要：

The toxicity of 1,2,4-Trichlorobenzene （1,2,4-TCB） to adult zebrafish and embryo was studied by static test method. Several enzyme activities, such as superoxide dismutase （SOD）, catalase （CAT） and alkaline phosphatase （AKP）, in adult zebrafish and embryo under the exposure of different concentrate 1,2,4-TCB were de tected to analyze the toxicant mechanism. Results showed that low concentrate 1,2,4 TCB （2, 4, 8 mg/L） had cer- tain stress effect on SOD activity; the SOD activity in treatment groups were higher than that in control groups on 8 d post-exposure. High concentrate 1,2,4 TCB （16 mg/L） had significant inhibition effect on SOD activity. CAT activi- ties in 4 mg/L treatment groups had significant difference with control groups during 4-10 d; CAT activities in 2, 4, 8 mg/L treatment groups was increased first and then decreased with prolonging the exposure time. AKP activity presented no significant difference with control group on 4 d post exposure, while significant difference of AKP activi- ty were found in 2 and 4 mg/L treatment groups on 8 d. The activities of AKP in 16 mg/L exposure were significantly lower than that of control groups. The zebrafish embryos exposed to 1,2,4-TCB were studied after hatching for 6 d. The proteins in embryos were no significantly activated except 3.46 mg/L treatment groups. The activities of SOD, CAT and AKP in treatment groups showed no significant difference with control groups. The embryo was more sensi- tive to 1,2,4-TCB than adult zebrafish.