中文摘要：

蛋白质酪氨酸硝化是一种重要的蛋白质翻译后的选择性修饰，其产物3-硝基酪氨酸可以作为检测细胞和组织损伤的一个生物标志。本文详细介绍了分析检测蛋白质酪氨酸硝化的各种方法，主要包括免疫法、分光光度法、色谱法、质谱法和电泳法等，并对其检测方法的发展趋势进行了评述和展望。

英文摘要：

Protein tyrosine nitration is an important selective post-translational modification, and the product, 3- nitrotyrosine, has been utilized as a biomarker of tissue and cell injury. In this review, the determination approaches of the analysis of 3-nitrotyrosine, including immunoassay, spectrophotometry, chromatography , mass spectrometry and capillary electrophoresis are systematically discussed. The developing trend of protein tyrosine nitration detection is also proposed.