中文摘要：

利用毛细管胶束电动色谱在线高盐堆积技术研究NaNOz-H202-氯高铁原卟啉（hemin）-酪氨酸（tyro-sine）体系中3-硝基酪氨酸（3-NT）的测定，建立了一种新的测定生物样品中微量3-NT的方法。最佳分离条件为：含SDS（30mmol／L）的Na2B407，（36mmol／L）缓冲液（pH9．3），进样压力为3．4kPa，进样时间为30s，毛细管柱温度25℃，分离电压20kV，样品中NaCl100mmol／L。在此条件下，3-NT检出限为0．1μmoL／L（S／N=3）；线性范围为0．78～50ixmol／L；线性相关系数为0．9953。保留时间日内与日间的RSD（n=5）分别为1．6％和2．8％，加标回收率为95．2％～103．1％；样品不同浓度添加水平的日内和日间的RSD（n=5）均小于3％。本方法简单、快速、灵敏度高，为直接分析生物样品中的微量3-NT提供了方法。

英文摘要：

A novel method for the quantification of minor 3-nitrotyrosine （3-NT） in NaNO2-H202-hemin-tyro- sine system was established by micellar electrokinetic chromatography with on-column high-salt stacking. The optimum CE separation conditions were achieved by using 36 mmol/L of Na2B40v as the running buffer contai- ning 30 mmol/L of SDS at pH 9.3, injection time of 30 s at pressure of 3.4 kPa ; separation voltage of 20 kV at temperature of 25℃ ; sample matrix containing 100 mmol/L of NaC1. Under the optimal CE conditions, the linear relationship ranged from 0.78 to 50 ixmol/L （ r = 0. 9953 ）, and the limit of detection （LOD） was 0.1 Ixmol/L （S/N = 3）. The RSDs of migration time for inter- and intra-day were 1.6% and 2. 8% , respectively. The recoveries were 95.2% - 103.1% and the RSDs for inter- and intra-day were less than 3% at various spiked levels. In this study, useful method is provided for the directly analysis of minor 3-NT in biological samples. The method is simple, rapid and sensitive.