中文摘要：

为筛选北京油鸡和来航鸡脾脏组织中与抗病力差异相关的候选基因和信号通路,本研究采用高通量测序技术,构建了42日龄北京油鸡（抗病力较强）和来航鸡（抗病力相对较弱）脾脏组织的数字基因表达谱,对差异表达基因进行了GO功能分类、KEGG信号通路分析和STRING互作网络构建,并利用荧光定量PCR验证了部分差异表达基因。结果表明,以来航鸡为对照组,北京油鸡与之相比,差异倍数在2倍以上的共有1335个基因,其中693个上调表达,643个下调表达,包括多个与淋巴细胞的激活、分化和增殖、免疫器官发育等相关的基因,主要涉及到结合功能、细胞组成、细胞加工和生物学调节等功能。对通路显著性分析发现,与免疫相关生物学通路共有4个,其中BCR信号和TLR信号涉及脾脏B细胞介导的体液免疫应答反应,两条通路上的差异表达基因构成1个相互连接的互作网络,其中D79B、CD44、IL1B、SOCS1及TLR4等位于重要节点位置。为今后挖掘新基因和研究影响鸡抗病力的遗传因素等提供理论依据。

英文摘要：

To screen the differentially expressed genes and pathway related to anti-disease activity in the spleen of chickens with different disease resistance. The digital gene expression （DGE） pro- file in Beijing fat chicken （strong disease resistance） and Leghorns （low disease resistance） at 42 days of age were built by high-throughout sequencing technology, the different expression genes were screened and annotation. Significant enrichment analysis of GO and Pathway were done,and the protein interactions network analysis for the differentially expressed genes was also built by STRING soft. Several differently expressed genes were verified with real time quantitative PCR. The results showed that Beijing fat chicken compared to Leghorns, different multiples more than two times were 757 genes,among them,334 were up-regulated,and 423 were down-regulated. Dif- ferent expression genes included some genes related to T cell and B cell activation,differentiation, proliferation and immune organ development, etc. These differentially expressed genes were also progress, biological regulation, etc. The pathway involved in binding functions, cell component, cell ~ analysis suggested that four were related to Immune,among which B cell receptor signaling path- way and Toll-like receptor signaling pathway were involved in the humoral immune response mediated by B cells. These differential expression genes of two pathways constitute a connected in- teraction network, and CD79B, CD44, ILIB , SOCS1 and TLR4 were located in an important node position. The results of this study could provide a theoretical basis for the novel genes involved in spleen performance,and for further investigating the functions and the regulatory mechanisms of these genes in anti-disease activity.