这研究被做在中国决定梨花和芽强风的原因的有机体。它被细菌学的测试识别,电镀物品显微镜的观察, Koch 的要求测试, Biolog,脂肪酸甲基酉旨(名声) ,和反应(PCR ) 测试,并且与标准相比引用的聚合酶链拉紧。六代表性的紧张从 20 病原细菌从 16 件 diseased 样品孤立显示出类似于 Pseudomonas syringae pv 的三标准紧张的特征。从比利时的 syringae。他们作为 P 被识别。syringae pv。有 0.57-0.86 的 Biolog 类似和 0.58-0.81 的名声类似的 syringae。细菌从征兆的植物和花被重新孤立。鉴定作为 P。syringae pv。syringae 被证实由用 PCR 教材和顺序测试,并且与相比上述结果。数据支持了在中国的梨花和芽强风能被 P 引起的事实。syringae pv。syringae。
This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch's postulate test, Biolog, fatty acid methyl esters (FAMEs), and a polymerase chain reaction (PCR) test, and compared with the standard reference strains. Six representative strains out of 20 pathogenic bacterial isolates from 16 diseased samples showed characteristics similar to three standard strains of Pseudomonas syringae pv. syringae from Belgium. They were identified as P. syringae pv. syringae with a Biolog similarity of 0.57-0.86 and FAMEs similarity of 0.58-0.81. The bacterium was reisolated from the symptomatic plants and blossoms. Identification as P. syringae pv. syringae was confirmed by using PCR primers and sequence tests, and compared with the above-mentioned results. The data supported the fact that the pear blossom and bud blast in China could be caused by P. syringae pv. syringae.