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Pear Blossom Blast Caused by Pseudomonas syringae pv. syringae in China
  • ISSN号:1001-7216
  • 期刊名称:《中国水稻科学》
  • 时间:0
  • 分类:S436.612[农业科学—农业昆虫与害虫防治;农业科学—植物保护]
  • 作者机构:[1]Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R.China, [2]Zhejiang Provincial Agricultural Bureau, Hangzhou 310029, P.R.China, [3]Hangzhou Plant Protection Station, Hangzhou 310020, P.R.China
  • 相关基金:This paper is translated from its Chinese version in Scientia Agricultura Sinica.We thank Li Junying from Zhejiang University for elec- tron microscopy assistance; Wang Xiao, Fang Yuan, Lou Miaomiao, and Zhu Bo from Zhejiang University for their assistance; Jean Swings from Ghent University in Belgium and Liu Bo from Fujian Academy of Agricultural Sciences in China for providing standard strains. This work was supported by the National High Technology Research and Development Program of China (2006AA 10A211), National Natural Science Foun- dation of China (30671397), and Hangzhou Agricultural Development Foundation, China (2007-2008).
中文摘要:

这研究被做在中国决定梨花和芽强风的原因的有机体。它被细菌学的测试识别,电镀物品显微镜的观察, Koch 的要求测试, Biolog,脂肪酸甲基酉旨(名声) ,和反应(PCR ) 测试,并且与标准相比引用的聚合酶链拉紧。六代表性的紧张从 20 病原细菌从 16 件 diseased 样品孤立显示出类似于 Pseudomonas syringae pv 的三标准紧张的特征。从比利时的 syringae。他们作为 P 被识别。syringae pv。有 0.57-0.86 的 Biolog 类似和 0.58-0.81 的名声类似的 syringae。细菌从征兆的植物和花被重新孤立。鉴定作为 P。syringae pv。syringae 被证实由用 PCR 教材和顺序测试,并且与相比上述结果。数据支持了在中国的梨花和芽强风能被 P 引起的事实。syringae pv。syringae。

英文摘要:

This study was done to determine the causal organism of the pear blossom and bud blast in China. It was identified by a bacteriological test, electro-microscopic observation, Koch's postulate test, Biolog, fatty acid methyl esters (FAMEs), and a polymerase chain reaction (PCR) test, and compared with the standard reference strains. Six representative strains out of 20 pathogenic bacterial isolates from 16 diseased samples showed characteristics similar to three standard strains of Pseudomonas syringae pv. syringae from Belgium. They were identified as P. syringae pv. syringae with a Biolog similarity of 0.57-0.86 and FAMEs similarity of 0.58-0.81. The bacterium was reisolated from the symptomatic plants and blossoms. Identification as P. syringae pv. syringae was confirmed by using PCR primers and sequence tests, and compared with the above-mentioned results. The data supported the fact that the pear blossom and bud blast in China could be caused by P. syringae pv. syringae.

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期刊信息
  • 《中国水稻科学》
  • 北大核心期刊(2011版)
  • 主管单位:农业部
  • 主办单位:中国水稻研究所
  • 主编:程式华
  • 地址:杭州市体育场路359号中国水稻研究所内
  • 邮编:310006
  • 邮箱:cjrs@263.net
  • 电话:0571-63370278
  • 国际标准刊号:ISSN:1001-7216
  • 国内统一刊号:ISSN:33-1146/S
  • 邮发代号:32-94
  • 获奖情况:
  • 首届全国科技期刊评比二等奖,第二届全国优秀科技期刊评比三等奖,农业部浙江省优秀期刊,国家期刊奖百种重点期刊,百种中国杰出学术期刊,中国精品科技期刊,中国国际影响力优秀学术期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,美国生物科学数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:17826