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水稻细菌性褐条病病原的鉴定
  • 期刊名称:中国水稻科学
  • 时间:0
  • 页码:198-200
  • 语言:中文
  • 分类:Q939[生物学—微生物学] S435.111[农业科学—农业昆虫与害虫防治;农业科学—植物保护]
  • 作者机构:[1]浙江大学生物技术研究所水稻生物学国家重点实验室,浙江杭州310029
  • 相关基金:基金项目:国家自然科学基金资助项目(30671397).
  • 相关项目:水稻细菌性谷枯病菌检测技术研究
中文摘要:

为明确从褐条病稻苗上分离出来的病原细菌并与西瓜果斑病菌相区分,对该病原特性进行了研究。分离获得6株褐条病致病菌,其中4株经主要细菌学特性、菌落形态、致病性、Biolog、脂肪酸分析(FAME)、电镜观察、Nested-PCR鉴定及与3株水稻细菌性褐条病标准菌株和2株西瓜果斑病标准菌株的比较,证实了该病是由单极鞭革兰氏阴性细菌Aci-dovorax avenae ssp.avenae引起的。FAME将水稻细菌性褐条病菌误鉴定为西瓜果斑病菌,而用Biolog和nested-PCR鉴定能得到准确的鉴定结果。

英文摘要:

For elucidating the brown stripe pathogen of rice and differentiating it from watermelon blotch bacterium, four out of six bacterial strains isolated from the diseased rice seedlings were compared with three standard reference strains of Acidovorax avenae subsp, avenae and two standard reference strains of A. avenae subsp, citrulli by the bacteriological tests, col- ony morphology, pathogenicity, Biolog, FAME analysis, electromicroscope observation and nested-PCR. It has confirmed that A, avenae subsp, avenae is the causal agent of bacterial brown stripe of rice. The results also showed that FAME analysis misidentified some strains of A. avenae subsp, avenae as A. avenae subsp, citrulli whereas Biolog and the nested-PCR were the best way for distinguishing the two bacterial pathogens.

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