中文摘要：

为了探讨壬基酚（Nonylphenol,NP）对海洋微藻的生态毒性效应,实验选择三角褐指藻（Phaeodactylum triconutum Bohlin）作为受试对象,设置7个NP质量浓度梯度（0、0.2、0.4、0.6、0.8、1.0和2.0 mg.L-1）,测定了不同浓度处理下三角褐指藻的生长情况、光合色素含量、可溶性蛋白含量、丙二醛（MDA）含量及最大光能转化效率（Fv/Fm）以及24 h,48 h,72 h,96 hEC50等指标。结果表明,NP对三角褐指藻的96 h EC50为0.84 mg.L-1;当NP暴露浓度在0.4 mg.L-1以上时对三角褐指藻生长表现抑制效应,而且随着NP质量浓度的增加,生长抑制效应加强,当暴露浓度≥1.0mg.L-1时即可造成藻细胞大量死亡,当NP暴露浓度≥2.0mg.L-1时,藻细胞基本不能生长。三角褐指藻的细胞密度、可溶性蛋白含量、光合色素含量以及Fv/Fm等指标随NP暴露浓度质量浓度的增加而下降的幅度更加显著,暴露处理24 h,MDA含量随NP质量浓度的增加而上升,表明较高质量浓度的NP胁迫,使藻细胞膜脂过氧化程度增强,可能会导致细胞膜结构的破坏和功能的丧失。NP对三角褐指藻的生长具有明显的毒性效应,并可以造成抗氧化酶系统和光合系统的损伤。

英文摘要：

In order to explore the toxic effects of nonylphenol （NP） on marine microalgae, effects of NP on the growth of Phaeodactylum triconutum were studied. The exposure concentrations of NP were 0, 0.2, 0.4, 0.6, 0.8, 1.0 and 2.0 mg.L^-1. Growth, photosynthetic pigment contents, soluble protein contents, MDA contents and Fv/Fm, as well as 24h, 48h, 72h and 96h EC50 were measured during the experimental period. The results showed that, the 96 h EC50 of NP for P. triconutum was 0.84 mg.L^-1. When the exposure concentration of NP was above 0.4 mg.LI, the growth ofP. triconutum was inhibited. And when it was more than 1.0 mg·L^-1 there was mass mortality of the algal cells. No growth of P. triconutum was observed as it came to 2.0 mg.L1. The decreases of growth, photosynthetic pigment contents, soluble protein contents, MDA contents, Fv/Fm were more notable with the increasing exposure concentration of NP. After 24 h exposure treatment, MDA contents increased with the increase of concentration of NP, indicating that high NP concentration enhanced algal cell membrane lipid peroxidation, and thus led to the structural damage and functional loss of cell membrane structure. NP had obvious toxic effects on the growth of P. tricornutum, and antioxidant enzyme systems and photosynthetic system were damaged.