中文摘要：

分别以4种游离氨基酸——丙氨酸、甘氨酸、谷氨酸和天冬氨酸为唯一氮源,采用实验室一次性培养的方法,研究典型赤潮藻——东海原甲藻（Prorocentrum donghaiense）、球形棕囊藻（Phaeoecystis globosa）和米氏凯伦藻（Karenia mikimotoi）及常见种类中肋骨条藻（Skeletonema costatum）的生长特性、光合特征及对氨基酸的吸收动力学特征,以无机氮源硝氮、氨氮作为对照.研究结果表明,在无菌条件下,东海原甲藻和球形棕囊藻可以利用多种游离氨基酸快速生长,而米氏凯伦藻和中肋骨条藻在以游离氨基酸为唯一氮源条件下不能维持生长.东海原甲藻和球形棕囊藻在丙氨酸中的生长速率最高,天冬氨酸和谷氨酸次之,甘氨酸最低.氨基酸处理组的Fv/Fm值在培养后期降幅小、降速慢,且高于无机氮处理组.东海原甲藻和球形棕囊藻对丙氨酸的最大吸收速率和半饱和常数分别为3.32、0.41pmol·cell^-1·h^-1和6.99和20.54pmol·L^-1.因此,海洋微藻对游离氨基酸的吸收利用具有显著的种间差异,东海原甲藻和球形棕囊藻具有更广的氮营养生态位,在近海有机污染不断加剧的背景下,更容易形成优势甚至暴发赤潮.

英文摘要：

The growth dynamics, photosynthetic characteristics and uptake kinetics of three harmful algae, Prorocentrum donghaiense, Phaeoecystis globosa, Karenia mikimotoi and a common phytoplankton species Skeletonema costatum were studied in the laboratory. The four algae were isolated or collected from the coastal waters of China, with alanine, glycine, glutamate and aspartate used as its sole nitrogen source, respectively. Dissolved inorganic nitrogen nitrate and ammonium were used as controls. The results showed that P. donghaiense and P. globosa were able to utilize multiple free amino acids, while K. mikimotoi and S.costatum could not grow with amino acids as the sole nitrogen sources under axenic conditions. The specific growth rates of P. donghaiense and P. globosa were the highest when alanine acted as the sole nitrogen source, followed by glutamate, aspartate and glycine. Fv/Fm values of amino acid treatments declined slightly and slowly in the later growth stage, but still higher than inorganic nitrogen treatments. The maximum uptake rates of P. donghaiense and P. globosa for alanine were 3.32 and 0.41 pmol·cell^-1·h^-1,respectively, and the half saturation constants were 6.99 and 20.54 pmol·L^-1, respectively. Consequently, the capability of uptake and utilization for DFAAs were significantly different among marine microalgae. In the coastal waters with increasing organic pollution, some harmful phytoplankton species equipped with broader nitrogen niche, such as P. donghaiense and P. globosa may enhance their competition potential, and even form red tides under suitable environmental conditions.