中文摘要：

目的：研究表面活性蛋白A（SP_A）和表面活性蛋白-D（SP-D）在人正常肾组织的表达及表达部位。方法：收集5例正常的人肾组织，培养人肾小管上皮细胞（HK-2），同时收集5例正常的人肺组织作为对照。应用免疫组化技术检测SP～A和SP—D蛋白在人正常肾组织的表达部位；应用RT—PCR检测SP—A和sP—DmRNA在正常肾组织和HK-2细胞中的表达。结果：免疫组化方法检测到SP—A和SP—D主要表达在皮质部的近曲和远曲小管；RT—PCR结果显示，在人的正常。肾组织和HK-2细胞中存在SP—A和SP—DmRNA表达。结论：与肺组织有相同内胚层来源的。肾组织可表达SP—A和SP—D。

英文摘要：

Objective： To investigate the expressions and distribution of surfactant protein-A（SP-A） and SP-D in normal human kidney. Methods： The expressions of SP-A and SP-D genes in human renal tubule epithelial cells （HK-2） in vitro and in five normal human kidneys were detected by RTPCR, and the distribution of SP-A and SP-D proteins were assayed by immunohistochemistry in five normal human kidney tissues, and compared with those in normal human lung tissues. Results： Immunohistochemical staining showed the distributions of SP-A and SP-D proteins were in distal and proximal convoluted tubule of human normal renal tissue; SP-A and SP-D mRNA could be detected by RT-PCR in both normal human kidney and HK-2 cells. Conclusion： SP-A and SP-D proteins and mRNA may be expressed in normal human kidney.