中文摘要：

目的：探讨G蛋白偶联受体激酶结合蛋白1（GIT1）在骨折愈合过程中的作用。方法：应用GIT1基因敲除小鼠和同窝野生型小鼠制作股骨骨折模型,每组30只。造模成功后7、14、21 d,应用X线、micro-CT、PECAM1免疫荧光染色等方法分析骨痂体积及新生血管情况。结果：与野生型小鼠相比,GIT1基因敲除小鼠骨折愈合延迟,骨痂内新生血管的体积和数量减少。结论：GIT1基因缺失导致骨痂区新生血管减少,从而延迟骨折愈合。

英文摘要：

Objective：To investigate the role of G protein coupled receptor kinase 2（GRK2） interacting protein-1（GIT1） in bone fracture healing. Methods：Femur fractures in GIT1 knockout（GIT1KO） mice（n=30） and WT mice（n=30） were performed. X-ray,micro-computed tomography imaging（micro-CT）,immunofluorescence of PECAM1 were performed 7,14 and 21 days after fracture.Results：Fracture healing was delayed and blood vessel volume as well as number were decreased in GIT1 KO mice compared to those in WT controls. Conclusion：GIT1 is an important signaling participant in fracture healing,with gene ablationleading to reduced callus vascularity and reduced osteoclast number in the healing callus.