中文摘要：

利用实时荧光定量RT—PCR技术，构建了标准重组质粒，制备了标准曲线，对不同年龄金黄地鼠腹股沟浅淋巴结、脾、心、肝、肺和肾提取总RNA，反转录后进行PrP基因的表达定量。结果发现，淋巴组织呈现高的表达量，外周组织的表达量比较低；不同组织在不同年龄出现表达高峰。

英文摘要：

The standard plasmid and the standard curve were constructed to quantify the PrP gene expression by using the real-time fluorescent RT-PCR technique. Total RNA was isolated from spleen, inguihal shallow lymphoid node, heart, liver, lung and kidney of mice at different ages to quantify the gene expression after the reverse transcription. Results showed that high mRNA levels were present in the lymphatic tissue but low in peripheral organs. The expression peaks of the prion gene were different for the different tissues.