中文摘要：

Agouti基因在哺乳动物毛色形成过程中扮演着很重要的角色,通过对山羊Agouti基因序列测定发现第1内含子T128缺失位点,利用PCR-RFLP技术检测该位点在国内10个山羊品种中的多态性,并将其与毛色进行相关性分析。结果表明,T128位点存在2个等位基因A（不缺失）和B（缺失）,产生3种基因型AA、AB和BB,其中AB基因型在白色山羊品种中占优势（76.14%）,AA基因型在棕褐色山羊品种中频率较高（77.41%）,BB基因型是黑色山羊品种的优势基因型（87.92%）,推测该位点可能在山羊毛色形成过程中发挥一定作用。T128缺失位点在10个山羊品种的平均期望杂合度仅为0.471 8,基因流为0.319 0,遗传分化系数为0.439 3,表明这10个山羊群体间遗传分化程度较低,遗传多样性相对贫乏。

英文摘要：

Agouti gene plays an important role in the pigment synthesis of domestic animals. A SNP-T128 del was found by sequencing. The genetic diversity of T128 del in ten Chinese indigenous goat breeds was analyzed, as well as the relationship between the genotypes and the coat color by PCR-RFLP. Two alleles of A （non-del） and B （del）, three genotypes of AA, AB and BB were detected. The genotype AA was superior in brown coat color goat breeds （77.41%）; genotype AB existed mainly in white coat color goat breeds （76.14%） and the goat breeds with black coat color had BB genotype mostly （87.92%）, so it was deduced that the T128 del might has the effect on the pigment synthesis of goat. The expected heterozygosity, the gene flow and the mean coefficient of gene differentiation of T128 del among ten goat breeds were 0. 471 8, 0. 319 0 and 0. 439 3, respectively. The results indicated that most goat breeds investigated here were poor in genetic diversity at T128 del.