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共基质对10株细菌降解苯并芘的作用研究

ISSN号：1672-2043
期刊名称：《农业环境科学学报》
时间：0
分类：X172[环境科学与工程—环境科学]
作者机构：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110016, [2]中国科学院研究生院,北京100039, [3]俄罗斯科学院西伯利亚分院地壳研究所,伊尔库茨克布,俄罗斯
相关基金：国家自然科学基金资助项（20337010）;国家重点基础研究发展计划973项目（2004CB418506）;中俄自然资源与生态环境联合研究中心.

作者：苏丹[1,2], 李培军[1], 台培东[1], E. V. Verkhozina[3]

关键词： PAHS, 细菌, 生物降解, 苯并芘, 葡萄糖, 菲, 共基质, PAHs, bacteria strain, biodegradation, benzo[a]pyrene, glucose, phenanthrene, co-substrate

中文摘要：

从石油污染的污泥中分离驯化出10株细菌（SB01-SB10），利用生物摇床实验对其降解苯并芘（BaP）的效能进行试验，研究了有（或无）共基质（葡萄糖Glu，或菲PHE）对细菌降解BaP的影响，并采用ANOVA和Tukey多重比较进行分析。结果表明，（1）当以BaP为惟一碳源和能源且BaP初始浓度为50mg·L^-1时（MS1），SB01的降解率最高，5d可降解31．0％；以Glu为共代谢基质时（MS2），SB09的降解率最高，可达36．9％；以PHE为共代谢基质时（MS3），SB01对BaP的降解率为46．0％。（2）Glu对SB01、SB02、SB03、SB07、SB10降解BaP有抑制作用，对SB01抑制作用最明显，使SB01的降解率降低了13．1％，Glu对SB05，SB08降解率无明显促进或抑制作用。（3）PHE对细菌降解BaP均表现出促进作用，对SB01的促进作用最明显，使其降解率提高15．0％。（4）Glu对SB09的促进作用大于PHE的促进作用。而对SB06，PHE的促进作用大于Glu。

英文摘要：

Autochthonous bacteria strains （SB01-SB10） were isolated from a contaminated soil and studied for their potential to degrade ben- zo[a] pyrene, a five-ring polycyclic aromatic hydrocarbon（PAH）, as their only soure of carbon and energy （MS1） or using glucose （MS2） or phenanthrene （MS3） as their primary nutrient co-substrates. One-way ANOVA and Tukey multiple comparisons were used in analyzing the degradation rate of BaP by the ten bacteria strains in different liquid mineral basal medium. The results showed that the biodegradation rate of BaP by different bacteria strains in different medium had significant difference. （1） When the initial concentration of BaP was 50 mg·L^-1, 31.0% of BaP could be degraded by SB01 for 5 days in SM1, which was the highest in the ten different bacteria. In SM2, SB09 had the highest degradation rate for BaP, 36.9% for 5 days. However, 46.0% of BaP were removed by SB01 in SM3. （2） The degradation of BaP for SB01, SB02, SB03, SB07, and SB10 were inhibitioed by glucose, but no significant difference for that of SB05 and SB08. Biodegradation rate was decreased 13.1% for SB01using glucose as the primary nutrient co-substrate compared with in SM1. （3） The biodegradation rate of BaP by these ten bacteria strains were enhanced as phenanthrene as their primary nutrient co-metabolism. （4） For SB09, the biodegradation rate significantly increased by 19.3%using glucose as the primary nutrient co-substrate, more than that by glucose. But for SB06, the role of phenanthrene was higher than the glucose for increasing biosdegradation rate.

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