采用实验室模拟的方法研究了重金属汞对2个草甸棕壤和2个黑土脲酶特征参数的影响,结果表明,同一类型土壤,有机质含量与脲酶特征参数之间关系密切Hg明显抑制脲酶活性,二者之间呈显著(P〈0.05)或极显著(P〈0.01)对数负相关关系,根据相应方程计算得到4个供试土样生态剂量(ED50)值分别为45.75、5、41、10.81和12.41mg/kg。随Hg浓度增加,脲酶动力学参数Km、Vmax和Vmax/Km均减小,除个别处理外,Hg浓度与3参数之间均达到显著或极显著负相关,揭示出脲酶特征参数可从不同角度表征汞对土壤脲酶活性的影响,作用类型表现为典型的反竞争性抑制现象。
The relationship between the concentration of Hg and urease characteristic parameters in two meadow burozem and two phaeozem soils was investigated. Results show a close relationship between urease activity and organic matter content in the same type of soil. Hg inhibited soil urease activity obviously, and the relationship between HgC12 and urease activities can be described by logarithmic equations significantly (P 〈 0.05) or markedly significantly (P 〈 0.01). It can be calculated that ecological dose (EDs0)of HgC12 based on the inhibition of urease activity was equal to 45.75、 5.41、 10.81 andl2.41 mg kg^-1 according to corresponding equations. The characteristic parameters of soil urease, including urease activity, Michaelis constant (Km), maximum reaction velocity (Vmax), and Vmax/Km decreased with the increase of Hg concentration, and the correlation was significant with few exceptions. To some extent, characteristic parameters of urease can be used as indexes to characterize the soil contamination of Hg. Hg behaves as a typical uncompetitive inhibitor to soil urease in this experiment.