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中文摘要：
用人工合成的无色孔雀石绿—牛血清白蛋白（LMG-BSA）作为抗原免疫BALB/c小鼠,并利用杂交瘤技术建立了1株稳定分泌无色孔雀石绿（LMG）单克隆抗体的杂交瘤细胞2E6。经鉴定表明,杂交瘤细胞染色体数为（99.12±3.60）,间接竞争ELISA（ciELISA）显示LMG50%抑制质量浓度为20.39μg/L,诱生小鼠腹水的抗体效价达2.3×10^5。与水产养殖中常见的禁用激素和抗菌药物的交叉反应小,表明该单抗具有较好的敏感性和特异性。
英文摘要：
With synthesized leuco malachite green-bovine serum albumin （LMG-BSA） as antigen, BALB/c mice was immuned and 1 hybridoma cell line coded 2E6 secreting monoclonal antibody against leueo malachite green （LMG）stably was established by the hybridoma technique. The identification results showed that the number of chromosome of the hybridoma cell reached （99.12±3.6）.The IC50 value of LMG was 20.39 μg/L by competitive inhibition enzyme-linked immunosorbent assay （ciELISA） and the antibody titer inducing mice ascites was 2.3×10^5. The cross reaction between the monoclonal antibody and prohibited hormones and antibacterial drugs seen commonly in aquieuhure was less, which showed that the monoclonal antibody had better sensitivity and specificity.
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