中文摘要：

目的：观察不同浓度17β-雌二醇（E2β）处理时,人胃癌BGC823细胞生长情况以及雌激素受体ERα36表达的变化,探讨E2β在胃癌生长调节中的作用及相关机制。方法：BGC823细胞经10-10,10-11和10-12 mol/L浓度的E2β处理24h和48h。细胞生长通过WST1方法检测。RT-PCR,Western blot及灰度分析法检测ERα36mRNA水平及蛋白水平变化,其平均光密度值通过t检验分析,应用免疫荧光染色方法检测ERα36蛋白在细胞中的位置变化。结果：E2β可促进胃癌BGC823细胞生长,但随着其浓度的上升,E2β的促生长能力下降。E2β可促进ERα36mRNA表达,该促进作用具有浓度依赖性。E2β处理BGC823细胞24h时,ERα36蛋白表达上升,48h时,ERα36蛋白表达下降。ERα36蛋白定位于细胞膜,E2β对BGC823细胞ERα36蛋白定位无明显影响。结论：E2β可促进胃癌细胞BGC823生长。E2β-ERα36可能通过膜信号通路参与了胃癌细胞的生长调节。

英文摘要：

Objective： To study the effect of 17β-estradiol（E2β） at different concentrations on the proliferation and estrogen receptor α36（ERα36） expression in gastric cancer cell line BGC823,and to explore the role of E2β in the growth of gastric cancer cells.Methods： BGC823 cells was dealt with 10-10,10-11,and 10-12 mol/L E2β for 24 h and 48 h.Cell growth was assessed by WST1 assay.The mRNA and protein expressions of ERα36 were examined by RT-PCR and Western blot respectively.Average optical densities were detected and processed using Student＇s t test analysis.Immuno-fluorescence assay was used to examine ERα36 location in BGC823 cell.Results： E2β could promote the growth of BGC823 cells,but with increasing concentrations,its promoting growth effect decreased.E2β could promote ERα36 mRNA expression in a concentration-dependent manner.E2β could promote ERα36 protein expression in 24 hours,but inhibitory effect was found after 48 hours.The location of ERα36 had no change as treated with E2β.Conclusion： E2β could stimulate the growth of gastric cancer cell line BGC823.E2β-ERα36 may regulate the growth of gastric cancer by membrane signal pathway.