中文摘要：

目的：寻求从福尔马林固定的癌组织中提取RNA的合理方法.方法：运用Trizol法从福尔马林固定的胃癌组织中提取RNA,用分光光度计测定RNA的产量和纯度;通过RT-PCR对不同长度的管家基因-actin进行扩增.结果：100 bp和300 bp的-actin均能顺利扩增出特异性条带,500 bp的-actin未能扩增.结论：用Trizol法从福尔马林固定的癌组织中提取RNA是可行的;进行RT-PCR时应尽量设计小片段引物（100~300 bp）以获得更好的扩增效果.

英文摘要：

Objective：To find a reasonable method for RNA extraction from formalin-fixed cancer tissue.Methods：To extract RNA from the formalin-fixed gastric cancer tissue with Trizol method.RNA yield and purity were detected with spectrophotometer,and RNA quality were de-tected by RT-PCR of different length housekeeping gene-actin.Results：High purity RNA can be extracted from the formalin-fixed gastric cancer tissue with this method.RT-PCR results showed that the 100 bp and 300 bp-actin can be successfully amplified,and the 500 bp-actin failed to am-plify specific bands.Conclusion：With Trizol method extracting RNA from formalin-fixed cancer tissue is feasible.We should try to design the small fragments primers（100-300bp） in order to obtain a higher amplification efficiency while carrying on RT-PCR.