中文摘要：

目的评价脊髓神经元微小RNA-132（miR-132）在小鼠骨癌痛中的作用。方法雄性C3H／HeJ小鼠，4～6周龄，体重20～25g。第一部分实验C3H／HeJ雄性小鼠52只，采用随机数字表法，将其分为2组（n=26）：假手术组（S组）和骨癌痛组（BCP组）；第二部分实验C3H／HeJ雄性小鼠32只，采用随机数字表法，将其分为4组（n=8）：假手术＋鞘内注射无核酸酶水组（S＋Veh组）、骨癌痛＋鞘内注射无核酸酶水组（BCP＋Veh组）、骨癌痛＋鞘内注射阴性对照锁核酸组（BCP＋c组）和骨癌痛＋鞘内注射miR-132反义锁核酸组（BCP＋AS组）。采用右侧股骨远端骨髓腔注射2×10＾5个NCTC2472细胞的方法制备骨癌痛模型。BCP＋c组及BCP＋AS组于接种后14d，分别鞘内注射阴性对照锁核酸和miR-132反义锁核酸12pmol／5μl，S＋Veh组及BCP＋Veh组给予等容量无核酸酶水，连续7d，1次／d。S组和BCP组分别于接种前、接种后4、7、10、14、21d时测定机械缩足反应阈（MWT）和自发抬足次数（NSF），并测定脊髓水平miR-132表达；S＋Veh组、BCP＋Veh组、BCP＋C组和BCP＋AS组于接种后14d鞘内给药前、接种后15、16、17、18、19、20、21d时测定上述痛行为学指标。结果与s组比较，BCP组接种后7-21d时MWT降低，NSF增加，脊髓水平miR-132表达上调（P〈0．05）；与s＋Veh组比较，其他3组各时点MWT降低，NSF增加（P〈0．05）；与BCP＋Veh组比较，BCP＋AS组接种后16．21d时MWT升高，NSF减少（P〈0．05）。结论脊髓神经元miR-132参与了小鼠骨癌痛的形成和维持。

英文摘要：

Objective To evaluate the role of spinal neuronal microRNA-132 （miR-132） in a mouse model of bone cancer pain （BCP）. Methods Male C3H/HeJ mice, aged 4-6 weeks, weighing 20-25 g, were used in the study. The experiment was performed in 2 parts. In experimentⅠ, fifty-two male C3H/HeJ mice were randomly divided into 2 groups （n = 26 each） using a random number table： sham operation group （S group） and BCP group. In experiment Ⅱ , thirty-two male C3H/HeJ mice were randomly divided into 4 groups （ n = 8 each） using a random number table： sham operation ＋ intratheeal DNAse/RNAse-free water group （S ＋ Veh group）, BCP ＋ intratheeal DNAse/RNAse-free water group （BCP ＋ Veh group）, BCP ＋ intrathecal negative control locked nucleic acid （LN~） group （BCP ＋ C group） , and BCP ＋ intratheeal miR-132 antisense LNA group （BCP ＋ AS group）. BCP was induced by injecting 2 ×10^5 NCTC 2472 cells into the distal medullary cavity of the right tibia. In BCP ＋ C and BCP ＋ AS groups, negative control LNA and miR-132 antisense LNA 12 pmol/5 μ1 were intrathecally injected, respectively, once a day for 7 consecutive days starting from day 14 after inoculation. In S ＋ Veh and BCP ＋ Veh groups, DNAse/RNAse-free water 5 μl was intrathecally injected once a day for 7 consecutive days starting from day 14 after inoculation. In S and BCP groups, the paw withdrawal threshold to mechanical stimuli （MWT） and the number of spontaneous flinches （ NSF） were measured, and the expression ot miR-132 in spinal cord was detected before inoculation and on 4, 7, 10, 14 and 21 days after inoculation. In S ＋ Veh i BCP ＋ Veh, BCP ＋ C and BCP ＋ AS groups, the parameters mentioned above were measured at 14 days before inoculation and 15, 16, 17, 18, 19, 20 and 21 days after inoculation. Results Compared with S group, MWT was significantly decreased, NSF was increased and the expression of miR-132 was up-regulated on 7-21 days after inoculation in BCP group. Compared with S ＋ V