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中文摘要：
为研究非肌肉肌球蛋白II型重链A（NMHCII-A）羧基端在猪繁殖与呼吸综合征病毒（PRRSV）感染Marc-145细胞中的作用，本实验通过提取Marc-145细胞总RNA，RT-PCR方法扩增NMHCII-A羧基端930bp基因PRA，并进行核苷酸序列测定。将该基因连接到pEGFP-N1载体中，采用双酶切和PCR方法对重组质粒pEGFP-N1-PRA进行鉴定。将pEGFP．N1-PRA转染至Marc-145细胞后感染PRRSV，测定TCID50值及间接免疫荧光（IFA）检测PRA对PRRSV感染Marc-145细胞的影响。TCID50及IFA结果显示，与未转染的Marc-145细胞相比，转染pEGFP．N1-PRA的Marc．145细胞的PRRSV感染能力降低50％。本研究初步证明了PRA编码蛋白在PRRSV感染细胞中的作用，为PRRSV感染机制的研究提供了一定的依据。
英文摘要：
To investigate the function of C-terminus nonmuscle myosin heavy chain II-A （NMHC II -A） to porcine reproductive and respiratory syndrome virus （PRRSV） infection of Marc-145 cells, a part of NMHC II -A gene （PRA） from Marc-145 cells was amplified by RT-PCR and cloned into pEGFP-N1 vector to construct recombinant plasmid pEGFP-N1-PRA for PRA overexpression in Marc-t45 cells. The results showed that the repliccation of PRRSV in the pEGFP-N1-PRA transfected Marc-145 cells was decreased by 50% in comparison with non-transfected cells by TCID50 titration and immunofluorescent assay. These results showed PRA encoding protein could influent PRRSV infection to Marc-145 cells. This finding provide a new way for further understanding of PRRSV infection to permissive cells.
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