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中文摘要：
从蓝耳病疑似患猪病料中分离到一株猪繁殖与呼吸综合征病毒。试验采用细胞培养、RT-PCR、免疫荧光技术等进行病毒的分离和鉴定。结果表明：Marc145细胞接种出现典型的细胞病变,毒价为5.5×105 TCID50/m L,RT-PCR扩增出特异性长度为559bp的DNA片段,用PRRSV N蛋白单克隆抗体为一抗,羊抗小鼠Ig G-FITC为二抗进行免疫荧光检测,检测到接种病毒的Marc145细胞中特异性荧光信号。说明该分离株为猪繁殖与呼吸综合征病毒。
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